By Anthony S. Fauci, R. Ballieux
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Extra info for Antibody Production in Man: In Vitro Synthesis and Clinical Implications
In an extensive clinical evaluation of a child, who survived a generalized BCG infection, we however, observed an unexpected reactivity against PPD. Standard in vitro tests for cellular immunity were normal. But, when the appearance of PFC in culture after stimulation with PPD was studied in cells from this child, very high numbers of polyclonal antibody secreting cells were recorded. Subsequent to these findings, cells were obtained also from other children who had developed local BCG infections in connection with immunization.
Addition of unirradiated T cells had no net helper or suppressor effect. 01) of PFC responses was noted (Fig. 4). When these adherent cell-enriched, T cell-depleted suspensions were depleted of adherent cells (generally from 45% originally to 15% or less) by passage over a sephadex G-10 column, suppression was no longer noted (Fig. 4). Hence, there is contained in the T cell-depleted fraction of mononuclear cells from normal PFC responders an enriched population of adherent cells capable of suppressing PFC responses in co-cultures.
This treatment did not prevent the B cells in this T cell depleted fraction from making Ig when subsequently cultured with PWM in the presence of added helper T cells. 28 Anthony S. Fauci and Barton F. Haynes This provides evidence that the Con A activated suppressor cell is not an Ig producing B cell and is probably a low affinity E rosetting T cell. With regard to B cells, it was further demonstrated that the Con A activated suppressor cell was found in the CR~ and not the CR+ subpopulation of nonadherent cells.